Oferta FPI -PRE2023-VACUNAS ORALES CONTRA IPNV EN TRUCHA BASADAS EN ESPORAS DE B. SUBTILIS Y MICROPARTICULAS DE FLAGELINA (PID2022-140624OR-I00)

Contacto: casado en inia.csic.es
Eduardo Gómez Casado
Dpt. Biotecnología . INIA-CSIC
Requisitos 300 ETS
Preadmision a Doctorado

Summary

The biggest threat to the aquaculture industry is to face losses due to disease
outbreaks. Vaccines are the most useful and cost-
effective method to generate a good protection against pathogens. In spite of all
the progress achieved, approved vaccines for aquaculture
are still costly, cause stress due to fish handling, and are only relatively efficient.
Traditionally, vaccines stimulate a systemic immune
response with an intra-muscular or intra-peritoneal injection. However, the main
route of exposure to aquatic pathogens are the mucosal
epithelia, that have an associated lymphoid tissue (MALT) of which not all
response mechanisms are known in detail in the trout. Oral
vaccine administration together with feed seems to be the best method, because
it is more versatile for the immunization against a wider
range of pathogens, allows to vaccinate high numbers of fish at a time (mass
vaccination), and reduces fish stress and costs to the
minimal. In this sense, Bacillus subtilis and flagellin could be used in trout as
vaccine platforms delivered with feed. B. subtilis has the
capacity to produce spores and flagellin can form microspheres with alginate. Our
main hypothesis is that oral VP2 vaccines
delivered by Bacillus subtilis spores and flagellin can reach the intestine, being
adjuvant itself for mounting a strong mucosal and systemic,
humoral (antibodies) and cellular (B, Th1, Th2, Th17) immune response.